Mycorrhizal colonization in Leucaena leucocephala as influenced by different levels of nutrient application in an alfisol amended with biochar

A greenhouse investigation was conducted to determine the effect of biochar application on effectiveness of VAM symbiosis in Leucaena leucocephala at different nutrient levels. This investigation consisted of 24 treatments resulting from factorial combination of two levels of biochar, six levels of nutrient and two levels of Glomus aggregatum inoculation. Mycorrhizal colonization in roots of Leucaena was significantly higher the low nutrient environments and it reduced at higher nutrient levels. The results of this investigation exemplifies the significance of optimizing nutrient application rate as well as level of biochar amendment in order to derive maximum benefit from VAM symbiosis.