1Centre for Plant Molecular Biology and Biotechnology, Department of Biotechnology, Tamil Nadu Agricultural University, Coimbatore-641003, India. E-mail: gizachew.gh@gmail.com
2Centre for Plant Molecular Biology and Biotechnology, Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore-641003, India
3Centre for Plant Protection Studies, Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore-641003, India
4Centre for Plant Protection Studies, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore-641003, India
5Centre for Plant Breeding and Genetics, Department of Millets, Tamil Nadu Agricultural University, Coimbatore, 641003, India
Online published on 28 November, 2016.
Bacterial blight (BB) is one of the important rice diseases that cause significant yield losses. The bacterial blight resistance gene Xa33 provides broad spectrum resistance against Xoo isolates in India. We assessed ten SSR markers for parental polymorphism from the vicinity of the gene Xa33. A new SSR marker RMWR7.5 closely linked to Xa33 was identified in the population of the cross ‘CO43’/‘FBRI-15’. This marker along with RMWR7.6 was validated for their specificity and usefulness in breeding. We alsoobserved the expected marker allele pattern according to the phenotypic classification into resistant and susceptible genotypes in F2 and F3 populations. The results confirmed that the markers RMWR7.5 and RMWR7.6 are effective for selecting resistant plants, thus can be used for deployment of Xa33 into the rice breeding program.
Bacterial blight, microsatellite markers, Rice, Validation, Xa33 gene
