1Dept. of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat
2Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni-Solan-173 230, H.P
Online published on 26 December, 2017.
RAPD molecular markers were used to evaluate the genetic diversity in populations of Melia azedarach Linn. from different locations of Himachal Pradesh and Punjab. Out of the 17 primers used, 13 yielded polymorphic banding patterns. In total, 97 different DNA bands were reproducibly obtained and all showed 100% polymorphism. The polymorphisms were scored and used in band-sharing analysis to identify genetic relationships. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped all the 19 populations into three major groups. Similarity indices ranged from 0.24 to 0.92. The highest similarity coefficient detected between progenies of selected mother trees from S (Dhaulakuan) and S (Ghagas) and lowest in the 17 18 populations of S (Bharmar) and S (Bharatgarh), indicated that 8 12 drek germplasm within Himachal Pradesh and Punjab constitutes considerably of broad genetic base.
Genetic variation, Melia azedarach, Mothertree, polymorphism, Progeny, RAPD
