The symbiont Rhizobium sp. was isolated from root nodules of Vigna radiata cultivated in the field. The isolate was sub-cultured in Yeast Extract Mannitol Broth (YEM) that was supplemented with optimum concentration of N, P, K and Mg salts and three plant growth hormones (IAA, GA and kinetin) and their various combinations. The growth behaviour, nitrate reductase (NR) activity, nitrite reductase (NiR) activity, nitrogenase (N-ase) activity and soluble protein were found to be enhanced with treatment of N, P, K and Mg salts and plant growth regulators (IAA, GA and kinetin) and their various combinations at different hours (24 to 120h) after inoculation.
Growth hormone, Rhizobium, Nitrogen metabolism