A comparative study of the frequency and spectrum of chlorophyll mutations induced by gamma rays (10kR, 20kR, 30kR and 40 kR), EMS (0.1%, 0.2%, 0.3% and 0.4%) and their combination (20kR + 0.1% EMS, 20 kR + 0.2% EMS, 20kR + 0.3% EMS and 20kR + 0.4% EMS) in M generation was made in Vicia faba var. minor. Six different types of chlorophyll mutants viz. albina, chlorina, maculata, tigrina, virescence and xantha were identified in the treated populations. Chlorophyll mutation frequency was calculated on plant population basis. Frequency of chlorina mutants was highest followed by xantha and other types. Combination treatments in general proved to be most effective followed by EMS and gamma rays in inducing maximum frequency of chlorophyll mutations (gamma rays + EMS > EMS > gamma rays).
Vicia faba, gamma rays, EMS, Chlorophyll mutations
