The nodal explants of Tinospora cordifolia, after surface sterilization with 70% ethanol for 1 minute and 0.1% mercuric chloride for 5 minutes, have been cultured on nutrient medium containing Murashige and Skoog's salts, vitamins, surcrose and various concentrations and combinations of BAP and NAA. The nodal segments exhibit basal callusing after 2 weeks and emergence of shoots from the axillary bud after 3 weeks. The emerging shoots have been sub-cultured to a fresh medium with NAA (1 ppm) and BAP (2 ppm). After 2 weeks of first sub-culture, shoots emerged from the single shoot along with root. The sub-culture of the callus on medium containing BAP (2 ppm) and NAA (1 ppm) has resulted only in shoot differentiation.
Tinospora cordifolia, tissue culture, callus, shoot regeneration
