In-Vitro Multipication of Plantlets of Bacopa monnieri and their ex-vitro Growth

High frequency plant regeneration was achieved in Bacopa monnieri by leaf and nodal explant culture. Multiple shoot buds with green callus were initiated from leaf culture when Murashige and Skoog medium was supplemented with IB A and KN. Green callus induction with indefinite number of micro shoots and roots were observed on same primary medium. Optimum green embryogenic callus induction with plantlet formation was observed on medium supplemented with NAA when 2,4 -D grown leaf-derived callus was subcultured. Fully mature plantlets were investigated on same MS containing NAA medium. Direct plantlet regeneration without intervening callus on medium containing IAA and KN from leaf margin was the remarkable feature of the present study. Plantlets were transferred to polybags containing sterilized coco peat and acclimatization process was done. Survival rate yas 100% when the hardened plantlets were transplanted to soil.