Vegetos- An International Journal of Plant Research
  • Year: 2012
  • Volume: 25
  • Issue: 2

Regulation of Nitrate Reductase Activity by Abscisic Acid in Poplar (Populus deltoids) Leaves.

  • Author:
  • Ritika Mishra, Sanjay K. Garg, Rakesh Singh Sengar1,
  • Total Page Count: 5
  • Page Number: 57 to 61

1College of Biotechnology, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut-250 110

Department of Plant Science, M.J.P. Rohilkhand University, Bareilly.

*Corresponding author Email: rs.svbpatu@gmail.com

Online published on 4 March, 2013.

Abstract

Excised leaves from Populus deltoids incubated in half strength Hoagland's solution containing 10mM KNO3 as nitrogen source and 25 to 100μM Abscisic acid for 24hr. Nitrate reductase activity were increased at lower concentration (25 μM) of ABA. Different concentrations of calcium (0.1, 0.5 and 1.0mM) with 25μM ABA inhibits the NR activity at lower to higher (0.1 to 1.0mM) concentrations of calcium. Different concentrations of calcium chelator EDTA (0.1 to 1.0mM) and calcium blocker LaCl3 (1.0 to 10.0mM) along with calcium (1.0mM) also decreases NR activity. EDTA specially reduce the availability of extracellular Ca2+ ions and ABA increased the inhibitory effect of LaCl3.

Keywords

Abscisic acid, calcium, nitrate reductase, Populus deltoids