Nitella mirabilis from Assam: Morphology and Molecular Characterization using RAPD markers

Nitella has been observed to grow in different aquatic habitats during algal survey from various areas of Silchar and was taxonomically identified as Nitella mirabilis (O. Nordstedt ex J. Groves) R. D. Wood, 1965 (dioecious). The physico-chemical parameters of water were also analyzed. Using isolated and purified genomic DNA, RAPD band patterns were recorded by PCR amplification for molecular characterization of maacrophytic algae. The electrophoretic patterns were analyzed to provide diagnostic fingerprints for each sample based on RAPD markers. The DNA fingerprint produced consisted of identical RAPD patterns in all the tested samples collected from different localities.