1Department of microbiology, C.C.S. University, Meerut
2Department of Biotechnology, MIET, Meerut
3Department of Agriculture, Shobhit University, Meerut
4Department of Botany, DN (PG) College, Meerut
*Corresponding author: Department of microbiology, C.C.S. University, Meerut, India. E-mail: alkamicro20@gmail.com
Online published on 3 April, 2018.
Antibacterail activity of flower extract of Holarrhena pubescens (kurchi) was studied against some bacterial strains as Proteus vulgaris, Pseudomonas fluorescens, Salmonella typhi, Klebsiella pneumoniae, Shigella flexneri. Methanol, Ethanol and Benzene extracts of Holarrhena pubescens flowers were effective against bacterial strains whereas benzene extract showed maximum growth inhibition (76.34%) against Shigella flexneri bacteria at 100μl/ml which are drug resistance for Tabramycin, Kanamycin, Norfloxacin. Minimum inhibitory concentration of benzene extract was determined against Shigella flexneri bacteria at 3.12 × 10-5 μl/ml. Present study depicts good antibacterial activity of Holarrhena pubescens flower extract against Gram negative bacterial strains.
Holarrhena pubescens, Antibacterial activity, Zone of Inhibition, Disc diffusion method, Minimum Inhibitory Concentration