Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Androgenic and plant regeneration potential from anther culture were investigated in cauliflower genotypes under different incubation temperatures, sucrose concentrations and regeneration media compositions. Anthers were cultured on MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L NAA growth hormone with four different treatments of sucrose i.e. 3%, 5%, 10% and 13%. Maximum androgenesis has been observed with 10% sucrose concentration. Anthers of all the varieties were incubated under three different temperatures i.e. 25°C, 32°C and 35°C. Among these incubation temperatures, higher androgenesis was observed at 32°C incubation temperature. Callus were cultured on five shoot regeneration media compositions, among which maximum regeneration has been observed on MS media supplemented with 5.0 mg/L BAP and 1.0 mg/L NAA followed by MS media supplemented with 6.0 mg/L BAP and 1.0 mg/L NAA. Maximum root regeneration has been observed in !/MS medium supplemented with 1.0 mg/L IBA growth hormone followed by/MS medium supplemented with 0.4 mg/L IBA. In cytological examination, 20.3% of the regenerated plants were found to be haploid while 29.8% were spontaneous doubled haploids.