Journal of Immunology and Immunopathology

UGC CARE (Group 1)
  • Year: 2002
  • Volume: 4
  • Issue: 1&2

Comparision of detection limit of a nonradioactive labeled VP4 cDNA probe based dot blot hybridisation test and RNA-PAGE of cell culture grown bovine rotavirus

  • Author:
  • I. Mishra, Y.P. Grover, R. Pandey, Minakshi
  • Total Page Count: 7
  • DOI:
  • Page Number: 33 to 39

Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, 125 004, INDIA.

Abstract

A dot blot hybridistion test for bovine rotavirus is described using non-radioactive digoxigenin labeled VP4 eDNA probe. The sensitivity of the probe was compared with that of RNA polyacrylamide gel electrophoresis. The detection limit for rotavims RNA in a sample by VP4 eDNA probe was 37.5 times higher than RNA-PAGE. VP4 eDNA probe could detect as little as 2 ng of viral RNA and was highly specific also.

Keywords

Nucleic acid hybridization, RT-PCR, Bovine rotavirus, Digoxigenin labeled probe