Effect of Temperature, pH, Light and Incubation Periods on Growth, Sporulation, Biomass and Gibberellic acid Production

Gibberellafujikluroi (GF-52) was grown in Richard's broth medium at four different pH levels ranging from 4.0 to 7.0, three different lights i.e. normal to dark conditions and three different incubations periods i.e. 9–12 days. Excellent sporulation was observed at 30°C and pH 5.0. Fungal biomass production was maximum at 4^th day of incubation. The optimum pH for GA₃ production was found 5.0, normal light conditions (60 W bulb) produced highest GA₃ production (0.57 g/l) and 9 days of incubation period was found suitable for GA₃ production.