Annals of Plant Protection Sciences
  • Year: 2005
  • Volume: 13
  • Issue: 2

Bio-efficacy of an alpha amylase inhibitor from seeds of cowpea (Vigna unguiculata L.) against cereal pests

  • Author:
  • John Sheeja1, C.S. Chaudhari2, A.G. Chandele2, Sushma Sabharwal1
  • Total Page Count: 3
  • Page Number: 275 to 277

1Department of Chemistry, University of Pune, Pune - 411 007

2Department of Agri. Entomology, College of Agriculture, Pune - 411 007, India

*Corresponding author. E-mail: ssab@chem.unipune.ernet.in

Abstract

Effect of a proteinaceous alpha amylase inhibitor (AI) from seeds of cow pea (Vigna unguiculata) was tested in vitro against gut alpha amylases of Tribolium castaneum and Corcyra cephalonica. Feeding trials were carried out with second instar larvae of C. cephalonica. Varying concentrations of the AI (0.2–1% of the diet) were used in the diet and larval development monitored for a period of 25 days. The AI inhibited gut alpha amylases of both the larvae in vitro. Among the C. cephalonica larvae feeding on diet containing 1% AI, 50% mortality was recorded 7 days after feeding and 90% mortality after 22 days of feeding.

Keywords

Alpha amylase inhibitor, Corcyra cephalonica, Tribolium castaneum, Vigna unguiculata