Effect of Fungicides and Native Antagonists Against Pythium Aphanidermatum in Tobacco Nurseries

Native antagonistic microorganisms were isolated from the rhizosphere of damping-off affected tobacco nurseries and screened in vitro for the antagonistic action against Pythium aphanidermatum causing damping off disease in tobacco by dual culture and cell free culture filtrate techniques. Aspergillus niger, A. flavus, Trichoderma viride and Pseudomonas fluorescens were found to be potential antagonists against P. aphanidermatum. Among the fungicides tested copper hydroxychloride, bordeaux mixture, metalaxyl + mancozeb were found to be compatible with the antagonists.