*Department of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar-143 005, India.
Department of Zoology, Guru Nanak Dev University, Amritsar-143005, India.
Online published on 23 July, 2012.
Sterilized seeds of Brassica juncea cv. PBR91 treated, with different concentrations (10−11M, 10−9M, 10−7M) of 24-epibrassinolide, were allowed to germinate and 15 days old seedlings were then inoculated with 1000J2s/plant of M. incognita. Enzyme estimation was carried out for catalase, ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase and glutathione reductase in both roots and shoots of host plant. The results revealed that specific activities of CAT, APOX, GPOX, SOD and GR were enhanced in root extracts of infected plants 7 and 15 days after inoculation. After EBl treatment, the enzyme activity was enhanced in highest concentration but was suppressed in lower concentrations. However, in case of shoots, the specific activity of all enzymes was suppressed in nematode inoculated plants. Whereas, the specific activity was enhanced in almost all the concentrations after EBl treatment. The enzyme activities of all enzymes in both roots and shoots of inoculated and treated plants were higher in 7 DAI as compared to 15 DAI.
Enzyme activities, 24-epibrassinolide, Meloidogyne incognita
