Molecular variability of Rhizoctonia bataticola causing charcoal rot of Soybean using RAPD marker.

The genomic DNA of 21 isolates of Rhizoctonia bataticola isolated by fresh mycelium of fungus grown in Richard solution and subjected to amplification with RAPD markers for diversity analysis. Amplified product of RAPD were resolved by electrophoresis and photographed under gel documentation system. Randomly selected 8 decamer primer amplified 64 RAPD marker loci. Out of these 64 bands, 29 bands (45.3%) were polymorphic. Similarity coefficient values for 21 accessions of R. bataticola in RAPDs marker was 0.69 – 0.98.