In vitro selection of Ginger (Zingiber officinale Rosc.) elite lines resistant to Fusarium oxysporum f.sp. zingiberi

Elite selection of Zingiber officinale lines tolerant to Fusarium oxysporum f.sp. zingiberi was done by using culture filtrate of Fusarium as a selective media. Calli were obtained from root explants of ginger which were selected from in vitro cultures of ginger. Resistant lines were selected at 15% level of culture filtrate after two cycles of selection. The selected calli were multiplied on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l benzyl adenine (BA), 0.5 mg/l 2,4 di chlorophenoxy acetic acid (2,4-D) and further used for plant regeneration. Shoot differentiation and regeneration from callus was achieved on MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l napthalene acetic acid (NAA). Roots were developed on same MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l NAA after 2–3 passages of subculture. A total number of 10 plants were regenerated from selected calli out of which three resistant plants were obtained after in vitro testing.