Patho-physiological studies on culture filtrates of Drechslera graminea causing leaf stripe of Barley

Barley leaf stripe caused by Drechslera graminea appeared in field as a small yellow leaf spots on leaves and sheath, later on extended into linear pale stripes on whole leaf and leaf became necrotic. The fungus produced maximum mycelium, sporulation and disease symptom development on Richard's medium followed by Sabourd's and Potato dextrose media. Maximum mycelium and sporulation were obtained at 25±2 ºC followed by 30±2 ºC and pH 6.0. The higher pH levels were unsuitable for symptoms development. The toxin treated seeds had much reduced germination compared to untreated control. The seedling vigour of barley was harmed by the effect of toxic metabolites produced by the test fungus.