Annals of Plant Protection Sciences
  • Year: 2015
  • Volume: 23
  • Issue: 2

Genetic diversity of Metarhizium anisopliae by RAPD primers

  • Author:
  • Joyarani Pegu, Pranab Dutta, A. Das, K.C. Puzari
  • Total Page Count: 4
  • Page Number: 347 to 350

Department of Plant Pathology, Assam Agricultural University, Jorhat-785 013, India

* pegujoyarani@gmail.com

Online published on 29 September, 2015.

Abstract

RAPD data analysis of Metarhizium anisopliae isolates recorded a total of 242 bands, of which 127 were monomorphic and 115 were polymorphic giving a polymorphism percentage of 47.5. Out of the 12 RAPD primers, RAPD 5 and RAPD 9 amplified the highest amount of bands (27 bands each). The number of fragments generated by each primer varied from 8 to 27. The primer RAPD 11 gave highest polymorphism percentage of 70.0% followed by RAPD 4 (65.2%). Lowest polymorphism percentage of 25.0% was recorded in RAPD 8.

Keywords

Metarhizium anisopliae, Molecular characterization, RAPD