Annals of Plant Protection Sciences

  • Year: 2015
  • Volume: 23
  • Issue: 2

Evaluation of culture filtrates of Trichoderma spp. on egg hatching and juveniles mortality of Root knot nematode

  • Author:
  • Kalpana Singh, Mukesh Srivastava, Anuradha Singh, Kusum Dwivedi, Yatendra Srivastava, Ved Ratan
  • Total Page Count: 4
  • DOI:
  • Page Number: 377 to 380

Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur-208 002, India

Abstract

Indigenous isolates of Trichoderma sp. (T. harzianum, T. viride) were evaluated against egg hatching and larval mortality of Meloidogyne incognita. These bioagent assayed for production of compounds active against egg masses of M. incognita, obtained from infected tomato roots. It was estimated from the result that showed Trichoderma harzianum was found more effective as 97% unhatched eggs were observed in standard concentration (T1-1: 10) in comparison to 94% in T.viride. The percentage was reduced gradually as the dilution was increased and it was 14% in T.harzianum and 16% in T. viride at 1: 100 dilution. Similarly in case of mortality percentage of juveniles, T. harzianum was more effective than T. viride at various time intervals, T. harzianum showed 100% mortality after 12 hrs in comparison to T. viride (90%). The mortality percentage was decreased as the dilution was increased in both the cases, but as the time of exposure in filtrate mortality was from 12 to 96 hours. It was 40% in the maximum dilution (1: 100) in case of T.viride whereas 37% was in T. harzianum after 96 hrs of time exposure in culture filtrates. Thus, it is analysed that antinematodal compound produced in culture filtrates of T. harzianum and T.viride directly affected the hatching of M. incognita eggs and because of mortality of juveniles their penetration into the host root was restricted.

Keywords

Culture filtrates, Meloidogyne incognita, Trichoderma spp