Probable location identification of inserted tungro resistant gene in Rice cultivar

Tungro is one of the most severe virus diseases of rice (Oryza sativa L.) and is caused by joint infection of Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). The identification of the probable location of RTBV-O-Ds2 gene on rice backcross progeny has been conducted in the present investigation. Amplification was found in the recombination of O-Ds2 forward primer and RM3 Reverse primer. The amplicon was eluted, purified and sequenced. The sequence data showed similarity to the DNA chromosome 4 bac clone of rice. The initial data showed that O-Ds-2 has been inserted in chromosome 4 in cv. Khitish BC3F4 progeny. The successful transfer of an RNAi construct, consisting ORF IV of RTBV in sense and antisense orientations was achieved from a transgenic PB-1 line into two high-yielding RTBV susceptible, popular rice cultivars (Khitish i.e. IET 4094; Shatabdi i.e. IET 4786).