Annals of Plant Protection Sciences

  • Year: 2019
  • Volume: 27
  • Issue: 3

Morphological and Molecular characterization of root knot nematode, Meloidogyne incognita associated with Cabbage and Cauliflower in Tamil Nadu

Department of Nematology, Tamil Nadu Agricultural University, Coimbatore-641 003

1Department of Vegetable Science, Horticultural College and Research Institute, T.N.A.U., Coimbatore-641 003

Abstract

The survey results revealed that Meloidogyne incognita was most prevalent in major cultivated regions. By using the morphometric analysis and molecular methods, M. incognita was identified. The ITS rDNA (Internal Transcribed Spacer region specific ribosomal DNA) was used for identification besides posterior cuticular pattern (PCP) of adult females and morphological characters of males and second stage juveniles also used for species level identification.

Keywords

Meloidogyne incognita, PCP, ITS rDNA sequencing