Isolation and identification of Trichoderma spp. through ITS-PCR from Chick pea growing areas of Andhra Pradesh

Trichoderma isolates isolated from different chick pea growing areas of Andhra Pradesh region were studied ITS-PCR. Collection of 16 isolates was further characterized into two main clades by internal transcribed spacer (ITS) sequence analysis. Consequently, a reliable phylogenetic tree was constructed containing isolates belonging to two groups. Group 1, the T. aspergillum, T. longibrachiatum Trichoderma spp. and Group II was outgroup includes T.viride, T. asperillum and T. asperelloides complex. The phylogenetic analysis showed many solitary clades of T.asperellum, which indicates that more diversity among the T. asperellum. Further, this analysis showed less bootstrap values indicates instability of the clade.