Studies on molecular identification of endorhizobacteria isolated from Fenugreek (Trigonella foenum-graecum L.)

Endorhizobacteria from Fenugreek root samples were isolated on nutrient agar medium, congo red yeast extract mannitol agar and pikovskaya medium. These isolated endorhizobacteria were purified through repeated subculture and were tested for biochemical identification. The molecular identification based on 16 S rRNA gene sequencing was done for the isolates. These endorhizobacteria Rhizo-fen-3, Rhizo-fen-8, Rhizo-fen-12 and Rhizo-fen-27 were identified as B. aryabhattai, B. megaterium, B. haynessi and Pseudomonas chengduensis, respectively through 16 S rDNA partial gene sequence homology. The finding revealed about the presence of other bacteria besides the well documented nodule forming bacteria Sinorhizobium melilotai from the root tissues of fenugreek crop grown in Rajasthan, India.