1Central Department of Botany, Patan Multiple Campus Patan Lalitpur, Patan, 44700, Nepal
*Corresponding Author: Belai Meeta Suwal Singh Central Department of Botany, Patan Multiple Campus Patan Lalitpur, Patan, 44700, Nepal. E-mail: belaisingh@gmail.com
Butea buteiformis is a valuable tree with large trifoliate leaves with beautiful flower. The seeds were surface sterilized and cultured on half strength Murashige & Skoog (1962) (MS) medium. Nodal explants obtained from cultured were subcultured on different concentrations of zeatin (Zin) and Indole-3-acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 5 μM Zin and 1.0 M IAA. After 8 weeks of culture the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.
Micropropagation, Murashige & Skoog (1962) medium, Nodal explants, Sand rooting, Acclimatization
