International Journal of Bioinformatics and Biological Science
Open Access
  • Year: 2013
  • Volume: 1
  • Issue: 1

Identification of microsatellite DNA markers in Labeo bata for genetic variability studies

  • Author:
  • Amrita Anand1, Gurudayal Ram1,, Pramod Washudev Ramteke2
  • Total Page Count: 10
  • Page Number: 27 to 36

1Department of Tissue Engineering, Jacob School of Biotechnology and Bioengineering, SHIATS, Allahabad, Uttar Pradesh

2Department of Biological Sciences, School of Basic Science, SHIATS, Allahabad, Uttar Pradesh

*Corresponding Author: Gurudayal Ram, Email: gurudayal.ram@shiats.edu.in

Online published on 25 April, 2013.

Abstract

Labeo bata, which belongs to the family Cyprinidae, has high economical importance and esteemed table fish worldwide. The present investigation identified six polymorphic microsatellite loci namely, Lr28, Lr29, Lr36, Lr38, Lro37 and Lro50 and two monomorphic loci Lro39 and MFW11. The observations showed that the primer from L. rohita and Cyprinus carpio, both belong to family Cyprinidae, could be used to identify microsatellite loci in L. bata. DNA sequencing of the identified microsatellite loci confirmed the presence of repeat motif.

Keywords

Microsatellite, DNA marker, Labeo bata