Molecular Characterization and Diversity Analysis of Soybean Varieties Against Soybean Rhizoctonia Aerial Blight Resistance

Analysis of polymorphism among soybean varieties and determination of genetic relationship among soybean varieties differing in Rhizoctonia Aerial Blight (RAB) resistance, using Inter Simple Sequence Repeats (ISSR) marker and Random Amplified Polymorphic DNA (RAPD). In the present investigation carried out 20 ISSR primers amplified 174 markers. Out of them 122 loci were polymorphic 70.11% (6.1±0.52 average), while 52 loci were monomorphic 29.89% (2.6±0.27 average) and RAPD primer amplified 78 loci, out of them 61 loci were polymorphic 78.02% (6.1±0.44 average), while 17 loci were monomorphic 21.78% (1.7±0.44 average). A dendrogram was generated by UPGMA clusters analysis based on Jacquard's similarity coefficient. Estimated genetic similarity among 18 soybean varieties using 20 ISSR primers, were 0.71 – 0.95. In this study, observed high level of polymorphism because low level of monomorphic band scored this type of markers system. Out of these 20 primers all of them produce polymorphic bands which represented maximum diversity in between soybean varieties. In these markers system to evaluate the genetic similarity as well as genetic relationships among the soybean cultivars, these were able to differentiate each and every varieties In the used of the ISSR primers which generated genetic similarity in between BRAGG with NRC 37 have 94% genetic similarity and was observed different cluster with NRC 37 and HIMSO 1602 with SL 710 and MACS 756 in between having about 93% genetic similarity, it was clustered with SL 710 and MACS 756 cultivars. In soybean cultivars using of RAPD primers e.g. PS 1374 with BRAGG and NRC 37 having with represented much closed clusters in which differentiate with other varieties.