Production parameter optimization for laccases by Aspergillus niger

Laccase belongs to the family of blue multi-copper oxidases (MCOS) that has three domain structures and usually contains four copper atoms. Aspergillus niger species are reported to produce laccase. An attempt was made to screening, optimization, production and partial purification of laccase enzyme produced from Aspergillus niger. Guaiacol and acetate buffer were used to assay laccase production. Laccase activity was highest when operated at the following conditions, 120 h incubation, 30°C temperature, pH-5, 2% glucose as carbon source and 2% peptoneas nitrogen source in the production medium. The enzyme was partially purified by ammonium sulfate precipitation and dialysis method.