Isolation and identification of Novel Lipase-producing Bacterium Aneurinibacillus aneurinilyticus LP-II

The aim of this research work was to isolate novel strain of lipase producer from local oil mill soil at Gangakhed. The lipase can be catalysing the transesterification of algae oil in to biodiesel. A sample of soil was collected from oil mill and enrichment the soil sample with basal lipase production media containing 1% Algae oil as a carbon source. Lipase producing microbes were screened on tributyrin agar plates, the activity of crude enzyme were determined. The isolate produced lipase and showed maximum activity 1.5 U/mL at 40^°C and at 7.0 PH, after 48 hours of incubation period at 150 rpm. Isolate identified by morphological, biochemical and 16s r-RNA sequencing gene. The 16s r-RNA sequencing revealed it is a new strain of Aneurinibacillus aneurinilyticus LP-II.