International Journal of Bioinformatics and Biological Science
  • Year: 2019
  • Volume: 7
  • Issue: 1and2

In silico Identification and Analysis of Stress Responsive Elements Present on Promoter Region of Acacia nilotica ALDH7 gene

1Department of Biotechnology and Bioinformatics, Deogiri College, Aurangabad, India

2School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

*Corresponding author: majadhav22g@gmail.com

Online published on 11 July, 2020.

Abstract

The promoter region of Acacia nilotica ALDH7 gene consists of 1233 bp and it belongs to linear DNA molecular class. A. nilotica consist a universal feature to response moisture stress and nutrient limitation. In A. nilotica ALDH7 gene promoter sequence, the nucleotide motifs were involved in respond to various stressful conditions, but which regulatory elements in ALDH7 gene respond positively under stressful conditions unknown to till date. In silico approaches were used to estimate the nucleotide motifs and its response to certain stressful condition and transcription activation located at ALDH7 gene promoter region. The result reveals that, ALDH7 gene promoter region includes six unique signature motifs such as ‘ACGTG’ is a high salinity and induction of dehydration stress-responsive putative cis-regulatory motif, the ‘TGAC’ is the biotic and abiotic stress and transcription activation responsive motif, the ‘CACGTG’ is the defense responsive motif, the ‘AAAG’ is Adenosine rich motif involved in carbon metabolism, the ‘GGATA & TAACAA’ motifs responsible for transcription activation. Thus, our analysis suggests that ALDH7 gene promoter region play an important role to healthy survival of A. nilotica under stressful conditions and provides medicinal and infrastructural products to fulfil the requirements of human being.

Keywords

Motif, Acacia nilotica, ALDH7, Biotic & abiotic stress, Promoter region, Transcriptional Activation