Effect of vermicompost and fertilizer on carbohydrate and functional indicator microbes

An incubation experiment was carried out at Dr. RPCAU, Pusa, department of soil science consisting of four levels of vermicompost (0, 1.25, 2.5 and 3.7 t ha⁻¹) and three levels of fertilizer doses (0, 100, 50 % RDF) in different combinations along with calcareous sandy loam soil in the incubation boxes to find out the effect of vermicompost and fertilizer on soil microbiological growth and to study about effect on acid hydrolysable carbohydrate content in incubated soil. For analysis of acid hydrolysable carbohydrate content 24N H₂SO₄ and 0.2% anthrone solution was used. The microbiological analysis was conducted using different media solution and after that serial plate dilution technique. Acid hydrolysable carbohydrate and all functional indicator microbes (Bacillus, Pseudomonas, Azotobacter, Azospirillum, Phosphate Solubilizing Bacteria, Starch hydrolyzing bacteria and Cellulose hydrolyzing bacteriaetc.) increased from 0^th DAI to 65^th DAI and then decreased from 65^th DAI to 115^th DAI. The combination of 3.75t ha⁻¹ of vermicompost and full dose 100% RDF was found superior among all other treatments. Combined application of vermicompost and fertilizer always provide better results in case of soil health and microbial studies.