Determination of In-vitro anticancer activity and short chain fatty acids in traditionally fermented millet gruels

The millets are called as nutricereals due to the availability of beneficial nutrients. The fermented millet gruels prepared from P. miliare, S. italica, P. scrobiculatum and E. frumantacea. and the millet gruels were analysed for the Short chain fatty acids (SCFA). The anticancer efficacy of the fermented millet gruels also investigated in vitro in the Human breast cancer cell line, MCF-7. P. miliare fermented gruel showed the acetic acid in the levels of 0.89 μM, lactic acid as 1.76 μM, propionic acid in the levels of 1.82 μM and butyric acids in the levels of 10.58 μM. The fermented S.italica gruel showed acetic, lactic and butyric acid levels of 1.12 μM, 2.41 μM and 42.85 μM respectively. The fermented P.scrobiculatum gruel showed the acetic acid of 1.04 μM, lactic acid of 0.89 μM, proponic acid of 2.26 μM and butyric acid of 3.09 μM. The fermented E. frumantacea also showed high levels of propionic acid (8.93 μM) and 2.7 μM of acetic acid. The anticancer efficacy also shown the S. italica fermented gruel have the high potential against the breast cancer cell line, MCF-7 compared to the other fermented millet gruel. It may be due to the high butyric acid content of the S. italica fermented gruel.