Aonla pomace as substrate for endoglucanase production using Trichoderma harzianum

Submerged fermentative (SmF) production of endoglucanase by a cellulolytic fungus Trichoderma harzianum any isolet number using aonla (Emblica officinalis Gaertn.) pomace as a substrate was standardized. Optimum production of the enzyme (0.57 ± 0.05 U/ml) was obtained at pH 4.0 and 30 ºC temperature using substrate concentration of 1.5% (w/v) supplemented with ammonium sulphate (1.5% w/w of substrate) as nitrogen source on 3^rd day of fermentation. The soluble extracellular protein was extracted and partially purified by acetone to an extent of 2.395 fold. Protein profiling on 12% Sodium dodecylsulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) revealed three bands with molecular size Corresponding to 86, 68 and 46 kDa, suggesting the isomeric forms of endoglucanase. The optimum endoglucanase activity in partially purified sample was achieved at pH 6.0 and 30 ºC temperature. The Michaelis-Menten constant (K_M) and V_max were found to be 13.89 mg/ml, and 0.215 μmole/min/mg, respectively.