International Journal of Innovative Horticulture
  • Year: 2014
  • Volume: 3
  • Issue: 1

Metabolite profiling of different developmental stages of Solanum lycopersicum L. by using gas chromatography-mass spectrometry (GC-MS)

  • Author:
  • Hema Bisht1, Deepa Bhagat1,, M.K. Bhatanagar2
  • Total Page Count: 12
  • Page Number: 41 to 52

1ICAR -National Bureau of Agriculturally Important Insects, Post Box No. 2491, H. A. Farm post, Bangalore-560024, Karnataka, India

2Pt Sambhoo Nath Shukla Government P.G. College, Shahdol, 484001, Madhya Pradesh, India

*Corresponding author: deepa.bhgt@gmail.com; Ph: +91-9902256372

Online published on 4 December, 2019.

Abstract

In the present study metabolomics variation in leaves and stems of Solanum lycopersicum L. (Tomato) has been assessed during the developmental stages using gas chromatography-mass spectrometry (GC-MS) techniques. Total three developmental stages of tomato plant were considered in present study. Twenty eight metabolites were detected in both in tomato leaves while and stems which includes mainly amino acid, fatty acids, polyol, vitamin E, organic acids, triterpene, and sterols. Each metabolite is represented by one or more ionic fragments of precise mass, which together can serve as a tag for that metabolite. Quantification of metabolites was done using its percentage peak area appeared at the total ion chromatogram in GC-MS analysis. These results lead to a better understanding of the plant metabolomics during plant aging and show the importance of plant developmental stage with respect to the nutritional profile of vegetables.

Keywords

Developmental stages, GC-MS, gas-chromatography-mass spectrometry, non-polar metabolites, Solanum lycopersicum