International Journal of Innovative Horticulture

In this study, a genotyping technique utilizing endosperm DNA sampled from Areca concinna Thwaites was optimized, which can be utilized as an alternative to leaf DNA-based genotyping for genetic studies and subsequent breeding applications. Extraction of DNA in pure form is important to undertake DNA based marker studies and optimization of extraction protocol is even more significant in members of Areca spp. with higher polyphenolic content. Embryo was scooped out from ripened fruit of A. concinna and endosperm was ground to a fine powder. SDS-based protocol did not yield DNA whereas genomic DNA could be extracted from endosperm with the addition of cellulase and mannitol to the extraction buffer. Amount of extracted DNA was more in protocol with cellulase in comparison to mannitol. The purity of the extracted DNA was validated by successful amplification of A. catechu-specific microsatellite markers. The protocol standardized could be applied to other large seeded plants. Also, DNA extraction from seeds facilitates longdistance transfer of plant materials (in the form of seeds) in comparison to leaf samples, which can perish faster unless kept on ice or are lyophilized.