Isolation of microbial contamination found in counterfeit eye cosmetic products

Eye cosmetics such as mascaras, eye shadows and eye liners are liable to microbial contamination prior to poor hygienic conditions either through preparation or distribution. This research aimed to isolate the microorganisms present in counterfeit eye cosmetic products. The isolations of microbial contamination from cosmetic products (n=5) were based on samples collected using convenience sampling method, then diluted with Tween80 and peptone water prior to isolation on growth media. Quantitative analysis was conducted using serial dilution method for total viable count and differential count whilst, qualitative analysis was done by identifying the isolate(s) against Gram staining. Antibiotic sensitivity testing was also conducted. Counterfeit eye cosmetic products of Brand A showed higher bacteriological count (95 CFU/g) compared to Brand B (no growth). Fungal and moulds count of counterfeit products of Brand A (11 CFU/g) was higher than Brand B (1 CFU/g). Microscopic observations showed higher numbers of gram negative bacilli compared to gram positive cocci. Antibiotic sensitivity testing showed high sensitivity towards Gentamicin and Penicillin V with zone of inhibition of 1.4 and 1.5 inches respectively. The higher microbial contamination in counterfeit eye cosmetic products compared to genuine eye cosmetic products suggest that the counterfeit products may have harmful effects on consumers with sensitive skin.