1Research Management Centre, Management & Science University, Malaysia
2Faculty of Health and Life Sciences, Management & Science University, Malaysia
*Corresponding Author: Dr. Mariam-Aisha Fatima Senior Lecturer Research Management Centre, Management & Science University, 40100, Shah Alam, Selangor, Malaysia. Email: mariam_aisha@msu.edu.my
Online published on 5 October, 2018.
Cytochrome P450 (CYP450) enzymes carry out oxidative metabolism of endogenous chemicals and this requires redox partner proteins to sequentially deliver electrons in electron transfer chain. Two redox partners that can be obtainedfrom Chinese spinach (Spinacia oleracia) areferredoxin (Fd) and ferredoxin NADP+ reductase (FNR). This study focuses on developing purification methods and identification of spinach redox partners and to study the capabilities of spinach redox partners to perform the role of reduction and oxidation. Single bands were visible on SDS-PAGE with sizes of-50 kDa and ∼8 kDa corresponding to FNR and Fd respectively. Spectral analysis yielded no flavorprotein or Fe-S cluster protein signature peaks.
Cytochrome P450, Spinach, Redox partner, Anion exchange chromatography, SDS-PAGE
