International Journal of Medical Toxicology & Legal Medicine
  • Year: 2021
  • Volume: 24
  • Issue: 1and2

Detection of rare variantallelesat Short Tandem Repeat (STR) loci in Indian population

1Research Scholar, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh

2Assistant Director, Biology, Forensic Science Laboratory, Rohini, Delhi, India

3Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India

4Professor, Dept. of Forensic Medicine & Toxicology, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India

*Corresponding Author: Dr. Amarnath Mishra, Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India, Email: amishra5@amity.edu, drmishraa1@gmail.com

Online Published on 22 December, 2021.

Abstract

In the present work DNA profiling of 310 person of Indian population was screened for rare alleles which were not included within the allelic ladder provided by the manufacturer of AmpFlSTR Identifiler Plus (Applied Biosystem) and Promega Power Plex 21 STR system (Promega Corporation)PCR amplification kits. A total of 2unusual rare alleles were detectedin Indian population by using Amp FlSTR Identifiler Plus (Applied Biosystem) and Promega Power Plex 21 STR system (Promega Corporation) PCR amplification kits. This study identified one variant allele at FGA locus (allele 22.2) and one at D1S1656 locus (allele 8). Allele 22.2 found at FGA fell within the allelic range while allele 8 found at D1S1656 fell prior to the allelic range.

Keywords

DNA Fingerprinting, STR, Rare Alleles, FGA, D1S1656