1Research Scholar,Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India
2Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India
3Professor, Dept. of Forensic Medicine & Toxicology, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India
*Corresponding Author: Dr. Amarnath Mishra, Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India, Email: amishra5@amity.edu, drmishraa1@gmail.com
Online Published on 22 December, 2021.
One of the primary objectives for conducting this study is to develop and modify the previously developed conditions of GC-FID instrument for qualitative determination of ketamine in forensic samples. The previously developed conditions of the instrument are modified to develop a new reliable method in GC-FID of Nucon model. A Column named AT-1000 was used to detect the standard of ketamine in methanol. The data is further interpreted by modifying the conditions of the instrument such as oven temperature, detector temperature, flow rate, injection temperature, injection volume and program rate to determine linearity, range, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ). The method shows accurate value of correlation coefficient which was (r2=0.997). The quantitative values of LOD & LOQ for the identification of ketamine were found 0.062ìg/ml& 0.187ìg/ml The recovery value attained spiking at 50% and 25% was 91.5%. The data is represented via charts, tables and graphs and modified conventional conditions of the instrument – GC-FID which is known to be most reliable and accurate proven instrument.
Ketamine, Forensic samples, Solid-phase microextraction, Blood, Viscera and injection, Gas Chromatography with Flame Ionization Detector (GC-FID)
