1Research Scholar,Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India
2Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India
3Professor, Dept. of Forensic Medicine & Toxicology, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India
*Corresponding Author: Dr. Amarnath Mishra, Assistant Professor and Program Leader, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India, Email: amishra5@amity.edu, drmishraa1@gmail.com
Online Published on 22 December, 2021.
A highly, specific, scientific, accurate and rapid method in instrument High - Performance Liquid Chromatography (HPLC) was used to identify the presence of Lignocaine drug in the forensic samples – blood, injection and viscera (liver). The extraction of Lignocaineis done by liquid – liquid method. In the following study, a new method is developed in HPLC under modified chromatographic conditions of the mobile phase consisting disodium hydrogen phosphate: methanol: triethylamine in the ratio of 50:50:0.1 (v/v/v) where the flow rate has been set at 1.2ml/min. The range of wavelength was set at 220nm. The assay exhibited peculiar linearity range (r2>0.997) via graph interpretation of area v/s concentration whose value ranges from 10-40ppm. The HPLC-UV method is one of the most reliable and analytical method in order to determine and to form calibration curve from linearity as well as to determine accuracy, precision, % RSD, robustness and detection of LOD & LOQ.
High - Performance Liquid Chromatography, Aesthetic drug, Lignocaine, forensic samples
