1School of Pharmacy, Management & Science University, 40100, Shah Alam, Selangor, Malaysia
*Corresponding Author: Dr. Wong Charng Choon, Senior lecturer, School of Pharmacy, Management & Science University, 40100Shah Alam, Selangor, Malaysia. Email: ccwong@msu.edu.my
Online published on 2 November, 2022.
Natural products in this case the weed shrub Chromolaenaodorata or Siam weed is investigated for its antibiotic effects in vitro. The methanolic extract alone has demonstrated the most antibacterial effect towards S. aureus and S. pyogene. Interestingly, synergistic antimicrobial effect in combination withmethanolic extract using tetracycline and ciprofloxacin was found in both Gram-positive and Gram-negative bacteria namely S. pyogene, and Klebsiella spp. for tetracycline (3.98 ± 0.24 cm; 2.61 ± 0.12 cm) and for Ciprofloxacin, S. aureus, S. pyogene, E. coli (3.28 ± 0.16 cm; 3.79 ± 0.07 cm; 4.37 ± 0.10 cm; p<0.05). Phytochemical screening revealed positive results for carbohydrate, protein, alkaloid, flavonoid and tannin as main constituents in C. odorata. It may seem that this weed may contain potent natural compounds for alternative antibiotic treatment or formulation as surface or sub-cutaneous creams.
Chromolaenaodorata L, Kirby–Bauer disk diffusion test, Minimum inhibitory concentration (MIC), Gram-positive, Gram-negative
