Molecular genetic techniques: In analysis of food contamination by lizard

In mass poisoning, detection of the nature of causative agent is important for management of trauma and forensic investigation. Most of the methods in clinical toxicology are developed for detection of toxins and poisons. The method of analysis for animal allergens responsible for mass disaster by severe allergies is yet to be developed. A RAPD-based method has been described for detection of species of animal from its morphologically unrecognizable fragments, recovered from food substances, consumption of which caused mass death. Pre-mixed RAPD reaction beak and six RAPD primers were used in PCR analysis. Among six RAPD primers used, any one of them was sufficient in resolving this practical forensic situation. This is the first report of a forensic application of RAPD DNA typing in identification of charred skeleton remnants of Lizardsp in food material. Further more unique amplicons were generated for different reptilian species, however no variations among individuals of same species were observed.