Effect of Pre-emergence Herbicides on the Activity of α-Amylase and Protease Enzyme during Germination in Pigeonpea and Carpetweed
Abstract
The effect of aqueous solution of trifluralin and pendimethalin at 0, 5, 10, 15 and 20 ppm on the activity of α-amylase and protease in pigeonpea and carpetweed (Trianthema portulacastrum L.) was studied in petridishes to understand the basis of selectivity of the herbicides towards weeds, Samples were taken for enzymes analysis at 6, 12, 24, 48 and 72 h after treatment. The activity of α-amylase and protease increased with passage of time during germination in pigeonpea and carpetweed. Increasing concentration of trifluralin and pendimethalin progressively reduced the activity of α-amylase and protease in pigeonpea and carpetweed. The reduction in the activity of α-amylase and protease was found to be more in pendimethalin than trifluralin during germination of the seeds in both the plant species.
