Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
*Corresponding author: Prof. S. Anupurba, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. Email: shampa_anupurba@yahoo.co.in
Online published on 17 June, 2017.
Tuberculosis is one of the major public health challenges globally. Extra pulmonary tuberculosis (EPTB) is defined as an isolated occurrence of TB at the body sites other than lungs. The aim of this study was to see the frequency of isolation of mycobacterium from different EPTB samples.
A total of 510 different extra pulmonary samples were collected during the period of August 2014 to July 2015. All the samples were subjected to Ziehl Neelsen staining. Samples were decontaminated by NALC-NaOH method and inoculated on a pair of Lowenstein-Jensen (LJ) media and one p-Nitrobenzoic acid (PNB). Cultures were incubated at 37°C and observed for 8weeks for any growth.
Mycobacteria were isolated from 60 samples of which the highest number of isolates was obtained from pus samples. Upon biochemical characterization, 6 isolates were characterized as non tuberculous mycobacteria. The frequency of isolation was 11.76%.
Our result suggests that due attention and emphasis should be given on diagnosis and management of EPTB.
Extra pulmonary tuberculosis, Mycobacterium tuberculosis, LJ culture
