1Department of Horticulture, Sri Venkatswara Agricultural College, Acharya N. G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India
2Department of Plant Pathology, Sri Venkatswara Agricultural College, Acharya N. G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India
3Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India
4Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi1100012, India
*Corresponding author e-mail: Madem Gurivi Reddy (gurivipath@gmail.com)
Online published on 25 August, 2022.
Symptoms of little leaf and flat stem were observed in Tinospora cordifolia plants at the medicinal block of the horticultural garden of S.V. Agricultural College, Tirupati, India. DNA was extracted from symptomatic and asymptomatic leaves and subjected to nested PCR assay with P1/P7 and R16F2n/R16R2 primers. The PCR amplified 1,250 bp product was eluted, sequenced and its analysis through phylogeny and virtual RFLP allows the identification of the presence of ‘Candidatus Phytoplasma australasia’.
Little leaf disease, 16SrII-D subgroup, Plant disease, Nested PCR, Sequencing
