Keladi Shivappa Nayaka University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka, India
*Corresponding author e-mail: Siksha Bhavana (bhavanas501@gmail.com)
Online published on 25 August, 2022.
Pigeon pea is an important pulse crop grown in tropical regions and mainly cultivated in semi-arid regions of India. Phytoplasma little leaf symptoms were observed on BGR-1 variety of pigeon pea in an experimental plot in Karnataka. The affected leaf samples were subjected to nucleic acid extraction for PCR amplification of phytoplasma 16S rDNA by nested PCR. The obtained product was sequenced and compared with the phytoplasma sequences available in the NCBI database. This sequence resulted having 100% nucleotide identity with a phytoplasma associated with sun hemp witches’ broom from Tirupati (GenBank accession number OL891522) placed in 16SrII group and with ‘Candidatus Phytoplasma aurantifolia’ that results therefore reported for the fist time in Karnataka state of India.
Cajanus cajan, Nested PCR, Phylogenetic analysis, 16S rRNA gene
