1Department of Plant Pathology, institute of Agriculture & Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India
2Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India
3Department of Plant Pathology, Sri Venkateswara Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India
*Corresponding author e-mail: Durgesh Kumar Dubey (durgesh2183@gmail.com)
Online Published on 19 April, 2023.
Murraya koenigii plants with leaf yellowing, witches’ broom and flat stem symptoms were observed during 2021-2022 in Gorakhpur and Ayodhya districts of Uttar Pradesh province, India. Detection of phytoplasmas in the symptomatic murraya plants was confirmed by nested PCR assays using the 16Sr RNA phytoplasma gene specific universal primers. The 16S rRNA gene sequence and RFLP analysis indicated that the phytoplasmas present in the leaf yellowing and witches’ broom affected murraya plants were enclosed in the 16SrII-C and 16SrII-D subgroups, respectively. This is the first report of 16SrII-C and 16SrII-D phytoplasmas presence in murraya plants in the world.
Curry leaves, Molecular detection, 16S rRNA gene
