1National Centre for Cell Science, SPPU Campus, Ganeshkhind, Pune, 411007, India
2Department of Microbiology, Tuljaram Chaturchand College, Baramati, Maharashtra, 413102, India
*Corresponding author e-mail: Amit Yadav (amityadav@nccs.res.in)
Online published on 23 May, 2023.
The inoculative potential ability of Recilia dorsalis and Exitianus indicus in soybean fields was detected through a feeding medium assay and TaqMan-quantitative PCR. The study inferred a seasonal pattern in detecting the phytoplasmas in the feeding medium. Phytoplasmas were consistently detected in the insect bodies throughout the season, suggesting also a possible role of alternate weed hosts as reservoirs. The PCR test of artificial diets provides an easy and reliable way to assess insect potential inoculative ability and enables large-scale testing.
Qpcr, Insect transmission, Seasonal phytoplasma pattern, Artificial diet
