Phytopathogenic Mollicutes
SCOPUS
  • Year: 2023
  • Volume: 13
  • Issue: 1

Multilocus RFLP characterization of ‘Candidatus Phytoplasma pyri’ strains in pear from Chile and Italy

  • Author:
  • Alan Zamorano1, Camila Gamboa1, Javiera Fuentes1, Sebastián Cabrera1, Camila Herrera1, Pietro Bianco2, Assunta Bertaccini2, Nicola Fiore1,*
  • Total Page Count: 2
  • Page Number: 79 to 80

1Universidad de Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, La Pintana, Santiago, Chile

2Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Italy

*Corresponding author e-mail: Nicola Fiore (nfiore@uchile.cl)

Online published on 23 May, 2023.

Abstract

‘Candidatus Phytoplasma pyri’ associated with pear decline in Italy and Chile severely affects orchards. To its molecular characterization, results of multilocus RFLP analyses on seven phytoplasma genes (16Sr, aceF, secY, leu, secA, tuf, and lsu 36p) resulted a promising tool. To complete this work and better understand the discrimination ability of the used genes the amplified DNAs obtained from Chilean and Italian strains will further be cloned and sequenced.

Keywords

Pear decline, Nested-PCR, Phytoplasma genes, 16SrX-C